The Department of European Art of the 16th–19th Centuries was established (as an independent department) in 1988. The Museum's Old Masters of Art collection, which includes 130 paintings and sculptures and 46 works on loan, is presented in six halls: four halls in the Museum's main building, and two halls dedicated to decorative art at the Helena Rubinstein Pavilion. The collection focuses on four areas: Italian 16th to 18th century art; Flemish and Dutch 16th and 17th century art; Jewish 19th century art; and decorative art: Gallé,
The collection includes works by Rubens, Van Dyck, Jan Breughel the Younger, Honthorst, Tenniers, Van Goyen, Flinck, Canaletto, Rigo, Reynolds, Maurycy Gottlieb, Jozef Israëls, Emil Gallé and others. The permanent collections are accompanied by temporary exhibitions, including: "Van Dyck and his Age", "Isaac Levitan", "Jozef Israëls", "Isidor Kaufmann", "Jan Lievens: The Sacrifice of Isaac", "Had Gadya: Passover Haggadahs", "Marvels of the East: Indian Paintings of the Mughal Period from the Nasser D. Khalili Collection", "Irises and Daffodils, Dragonflies and Butterflies: The Glassware of Emile Gallé", "Meissen Porcelain: The Danek and Jadzia Gertner Collection", "4,000 Years of Portraiture", "From Neo-Classicism to Romanticism", "Goya: Los Caprichos", "Rembrandt: 100 Etchings", "Old Testament, Old Masters: The Bible in 16th to 19th Century Art", "Femme Fatale", and others.